Molecular Characteristics of polycystic ovary syndrome by Real Time PCR. -

Martials and methods:Three ovarian normal women and Three ovarian tissue PCOS women through laparoscopic surgery in Hospital of Mansoura University, extraction total RNA from ovarian tissue (Extraction of total RNA by The IQeasy plus CTB RNA Extraction Mini Kit intron biotechnology, Korea). In addition, total RNA to cDNA by ((Synthesis cDNA by Maxima RT PreMix Kit, intron biotechnology, Korea.). Finally, preparation of reagent of the Real MOD Real time PCR Core Kit and Master Mix provides a system intron biotechnology, Korea. Reading by Real-time PCR performed with ABI Prism 7900 (Applied Biosystems). The study carried out to evaluate the effect of some genes that play role in the polycystic syndrome. Conclusion: There are some genes play important role in polycystic ovary syndrome as PDK4, NR4A1, SET and HIP-55 genes. Results:Pyruvate dehydrogenase kinase 4 (PDK4) and Serine transcription (SET) downregulated gene in normal women while upregulated in polycystic ovary syndrome. While NR4A1 (nuclear receptor subfamily 4, group A, member 1) and HIP-55 (src homology 3 domain-containing protein are upregulated gene in normal women while downregulated in polycystic ovary syndrome. - See more at: .