Study included using exogenous alloxan (100 mg
for every 1 kg of animal weight) for inducing experimental diabetes mellitus in
rats. 46 rats divided for two groups, during the time of study glucose,
glycogen levels estimated every week extend for 3 weeks. Also the study
included histo-pathological effect on liver and pancreas. The experiment showed
an increase in blood glucose, after 7 days, 14 days, 21 days of alloxan
injection, values were (11,92 ), (15,89), (17,31) mmol/Lrespectively, glucose
level of control group was (5.84 - 7.44 mmol/L). while glycogen level in
liverat the same period was (0,72 ), (018), (0.075) mg/g. Control group
glycogen was (3,18 ? 3.65mg/g) during 3 weeks of experiment. Histological
examination of liver samples of ratswere traced pronounced signs of toxic
hepatitis as a violation of the beam structure of the lobules, necrosis of
hepatocytes, fat and protein dystrophy, the presence of infiltration of
hematogenous cell clusters. In the control group was characterized by a uniform
distribution with respect to the same hepatocytes for liver slices, hepatocytes
and the structure of the test cells corresponded to the ratio of the classical
histological characteristics of the active functioning of the liver. The number
of β-cells in the islets of sharply reduced, in most of them marked
vacuolization of the cytoplasm, reducing the size of the nuclei, chromatin
condensation, in some cells - kariopik?noz. Presence of a lymphocytic
infiltrate along the periphery of the islets, interlobular connective tissue
edema, congestion of the capillaries; vascular stasis traced. - See more at:
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