Hypoglycemic and Antioxidant Effects of Zinc oxide nanoparticals in alloxan-Induced Diabetes Rats.

Zinc oxide nanoparticles (ZnONPs) of average diameter of 45 ? 5.0 nm were prepared using chemical reduction method and characterized by UV-Visible spectroscopy, Scanning electron microscopy (SEM), Atomic force microscopy (AFM), X-Ray diffraction (XRD) and Fourier Transmission Infrared spectroscopy (FTIR). To test the ability of ZnONPs to ameliorate antihyperglycemic and the oxidative stress status resulted in experimental diabetic rats induced by alloxan, sixty male albino rats with weight 220 ? 25 grams and age of 9 months were used in experimental design. Ten of them were served as control group and fifty rats were injected with alloxan at the single intraperitoneal dose of 150 mg/kg. Then, subdivided into, diabetic, diabetic rats + ZnONPs I, received single daily dose of 2.5 mg/kg b.w ZnONPs in suspension. Diabetic rats + ZnONPs II, received a single daily dose of 5.0mg/kg b.w ZnONPs in suspension , diabetic rats + ZnONPs III, received a single daily dose of 10 mg/kg b.w ZnONPs in suspension, diabetic rats + insulin; received a single daily subcutaneous dose of insulin 2U/kg b.w. At the end of experimental time(60 days) the blood glucose, serum insulin,glycoslated HbA1c, lipid peroxidation marker, malondialdehyde (MDA), reduced glutathione (GSH) and serum activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and Catalase (Cat) were determined. Results showed a significant alteration in the activities of SOD, GPx, CAT, MDA Insulin, HbA1c and FBS in animals treated of ZnONPs, compared with diabetic or diabetic + insulin group and their control group. The profound control of ZnONPs over the anti-oxidant enzymes in diabetic rats to normal, by inhibition of lipid peroxidation and reactive oxygen species generation during hyperglycemia evidence their antioxidant effect during diabetes. The administration of ZnONPs at 10 mg/kg b.w exhibited an insistent control over the blood glucose level, lipids and serum biochemical profiles in diabetic rats near to the control group provokes their effective role in controlling and increasing the organ functions for better utilization of blood glucose. Histopathological studies revealed the non-toxic and protective effect of the ZnONPs over the vital organs and can be used to ameliorate the hyperglycemia and oxidative stress status. - See more at: