The immune response of experimental animals against tetanus toxoid (TT)
vaccine was developed within 14 days post vaccination. Recorded data revealed
that antibody (Ab) level was time related, and the intraperitoneal (IP)
administration of vaccine showed a higher Ab level in a significant way than
subcutaneous (SC) one. Avidity index showed higher significant difference in
case of IP administration than SC one. The avidity index post intravenous
administration of anti-CD154 (MR1) monoclonal Ab was detected using ELISA technique
and germinal centers (GCs) were detected using immunofluorescence assay (IFA).
Histopathological examination revealed that the peaking of GCs was on the 10th
day post vaccination and an obvious deterioration was recorded on day 33 post
vaccination. It was found that the avidity index of serum Ab in MR1 injected
group was significantly lower than non MR1 injected group at day 69, suggesting
that GCs generate the precursors of high affinity BM AFCs. This finding
suggests that memory B cells and long-lived antibody secreting plasma cells may
represent independently regulated cell populations and may play different roles
in the maintenance of protective immunity. - See more at:
No comments:
Post a Comment