Saturday, 7 May 2016

ANTIBACTERIAL AND PHTYOCHEMICAL ANALYSIS ON BRASSICA OLERACEAE VAR ITALICA.

Objective: - To investigate the antibacterial activity and phytochemical analysis of Brassica oleraceae var. italica Methods: - The antibacterial activity was evaluated using agar well diffusion and microdilution methods against the bacterial (E.coli, Proteus, S.aureus, Klebsiella and Pseudomonas) iolates. The extraction of the vegetable was carried using the solvent namely acetone. Phytochemical, FT-IR and HPLC analysis was carried out in the acetone extract. Results:-Acetone extract showed maximum activity against E.coli, Proteus, S.aureus, Klebsiella and Pseudomonas. Phytochemical analysis of Brassica oleraceae extracts showed the presence of secondary metabolites like alkaloids, flavonoids, glycosides, phenolic compounds, saponins, tannins, terpenoids, carbohydrates and anthraquinone. The FT-IR and HPLC revealed different characteristic peak values with various functional compounds in the extracts. Conclusion: - From this study, it can be concluded that Brassica oleraceae var. italica exhibits antibacterial activity against certain microorganisms - See more at: 

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