Friday, 13 May 2016

BACTERICIDAL POTENTIALITY OF SELECTED BRYOPHYTES PLAGIOCHILA BEDDOMEI,LEUCOBRYUM BOWRINGII AND OCTOBLEPHARUM ALBIDUM.

A general lack of commercial value, small size, and inconspicuous place in the ecosystem have made the bryophytes appear to be of no use to common man. It is common knowledge that bryophytes are not infected by pathogens, even though most live in close proximity with the forest floor or organic decomposing substrates. Bryophytes contain numerous potentially useful compounds, including sugars, sugar alcohols, amino acids, fatty acids, aliphatic compounds, phenylquinones, flavonoids and phenolic substances, but much work remains to link medical efficacy with specific bryophyte species or compounds. Relatively little attention has been paid to correlate secondary metabolites of bryophytes with therapeutic uses. Hence the present investigation on selected bryophytes has been an attempt to screen the secondary metabolites and their potential roles as antibacterial. Initially, a phytochemical screening was attempted revealed a pool of secondary metabolites. Phenols and flavonoids were present in significant levels. Subsequently, phenols were fractionated revealed the presence of phenolic acids such as gallic, vanillic, p-hydroxybenzoic, ferulic, chlorogenic, sinapic, para coumarate and cinnamic acids. As the last phase, the antibacterial potentiality was analyzed. Methanolic extracts of the bryophytes strongly inhibited pathogenic microbial strains, including Salmonella typhimurium, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus, Bacillus subtilis, Proteus vulgaris, Pseudomonas aeruginosa as revealed by ELISA technique and disc diffusion method. Further, the bacterial survival-time analysis substantiated the MIC and MBC data. Further studies are warranted to analyze the mechanism of microbicidal action of the bryophytes. - See more at: 

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