Geophila repens, of the family Rubiaceae, is used as a hepatoprotectant
in the folk medical practices in Thiruvananthapuram district, Kerala,India.
This plant is also reported to possess several other medicinal properties.
Being restricted to specific pockets in under covers of forest areas, the
accessibility to this plant is quite difficult. Further, due to scarcity in
distribution there is a threat to its existence due to over exploitation,
urbanization and industrialization. In order to formulate effective
conservation strategies for frequently exploited plants, assessment of its
genetic diversity and population structure is urgent. Genetic diversity among
accessions of a plant can be analysed using various marker techniques.
Molecular characterization of the accessions of G. repens was done using RAPD
and ISSR markers. Random amplified polymorphic DNA (RAPD) markers and ISSR
markers are widely applicable because they are rapid, inexpensive, simple to
perform, do not require prior knowledge of DNA sequence and require very little
starting DNA template. A total of 11 accessions of G. repens were collected
from various localities, out of which 10 accessions were from Kerala and one
from Andaman islands. RAPD analysis was carried out using a set of 20 decamer
primers. ISSR analysis was also carried out in G. repens. The analysis was
carried out using a set of 12 primers. The effectiveness of the molecular
markers used for the characterization of Geophila was analysed at various
levels like polymorphism, Polymorphism information content (PIC) and resolving
power (Rp) and primer index values in the case of DNA based markers. This
reveals the efficiency of ISSR marker system compared to RAPD. - See more at
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