In this study, the infection of Begomovirus in Papaya (Carica papaya)
plants from New Delhi was suspected in area exhibiting leaf curl disease.
Rolling circle amplification and restriction fragment length polymorphism
analysis suggested that a begomovirus was present in symptomatic plants. The
full length sequence of a begomovirus DNA component was determined comprising
2745 nucleotides. Pairwise sequence comparison of the virus under study with
other begomoviruses, performed using the species demarcation tool, suggested
<92% pairwise nucleotide identity with Croton yellow vein mosaic virus
(CYVMV). Notably, no other genomic components, including satellites, were
obtained. Further nucleotide sequence comparison indicated its recombinant
origin. Infectious clone of the begomovirus was constructed and inoculated into
the leaves of Nicotiana benthamiana using a needleless syringe, and this
resulted in a mosaic pattern and slight curling of the leaves. - See more at:
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