Objective:- Hepatic injury and cell death in diabetes is a major health
problem worldwide. Oxidative stress is a contributing factor in diabetic
complications including liver. Various preclinical and clinicalreports have
indicated the antidiabetic effect of silymarin (SMN). The objective of this
study was to investigate the effect of SMN on oxidative stress and apoptosis in
liver of streptozotocin (STZ)-induced diabetes in rats. Methods-
:Streptozotocin (STZ, 50 mg/kg, ip) was injected into male rats, and after
diabetic induction SMN (80mg/kg) was orally administered for 21 days. Results:-
STZ produced remarkable hyperglycemia and a decrease in insulin level. This is
accompanied by increased alkaline phosphatase activity and bilirubin level with
remarkable decrease in albumin content in serum of diabetic rats. A significant
increase in oxidative stress was demonstrated by increased lipid peroxidation
with a decrease in the glutathione levels in liver of diabetic rats. Increased
percentages of apoptosis with down-regulation of Bcl-2 and activation of Bax as
well as the caspases-3 and 9 were demonstrated in the diabetic rats.
SMN-treated rats showed significantly lower levels of blood glucose,bilirubin
and alkaline phosphatase,and higher levels of insulin and albumin when compared
with diabetic group.SMN also prevented the increase in MDA and stimulated the
GSH production in liver of STZ-treated rats indicating improved hepatic redox
state. SMN-treated rats showed significantly higher level of Bcl-2 and lower
expression levels ofBax and the caspases-3 and 9 when compared with diabetic
rats. These effects might contribute and lead to control apoptosis in the liver
of diabetic condition. Conclusion:- SMN has an ability to protect liver by
modulating redox stateand apoptosis.These data indicate that SMN may be useful
as a therapeutic agent for liver injury in type 1 diabetes. - See more at:
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