In vitro grown microshoots of Sarcostemma brevistigma were used as
explant for encapsulation. Encapsulated microshoots were kept at 25°C, and the
success rate of regrowth was found to be approximately 50% following 1 months
of storage. Encapsulated microshoots showed 37.66% formation of multiple shoots
on plant growth regulator free Murashige and Skoog (MS) medium. Healthy root
formation was observed in all microshoots following two weeks of transfer on
half-strength MS medium containing 0.5 µM ?-naphthalene acetic acid and 0.5 µM
Indole acetic acid. These plants were subsequently transferred to pots
containing a mixture of soil, sand and farm yard manure (2:2:1, v/v), and then
shifted in the greenhouse. In the green house and the overall survival was
found to be 75% after 2 months. The genetic fidelity analysis of S. brevistigma
plants developed from encapsulated microshoots was done using random amplified
polymorphic DNA (RAPD) marker. For molecular analysis 25 decamer primers were
used to check genetic fidelity of plants selected from all three batches was
carried out using. Only 5 primers produced scorable amplified products and a
total of 38 bands were observed; out of these 10.52% bands were polymorphic.
Cluster analysis of the RAPD profile revealed an average similarity coefficient
of 0.95 confirming genetic stability of plants derived from encapsulated
microshoots following 1 months of storage at 25°C. - See more at: .
No comments:
Post a Comment