In pursuit of
selecting cheap raw material for producing lactic acid by fermentation ground
nut shells were chosen as substrate. However there is no much relevant
information was available on fermentation of ground nut shells. The
experimental studies on production of L lactic acid were explored, by solid
state fermentation. The experiments on groundnut shells were carried out, as
substrate which was one of the cheapest raw materials available in India. The
ground nut shells were pretreated with dilute acid (HCl&H2SO4), sodium
sulphite, NaOH and methanol solvent extraction methods for producing total
sugar. It was observed at temperatures ie., at 350C,370C and 420C, different
inoculums size(3ml to 6ml) and different pHs(4.5 to 5.5), the lactic acid
production is 12 g/l, `17 g/l and 23.0 g/l respectively. Lactic acid was
estimated by AC Kimberley Taylor method. The mutant cells were tested for its
stability on 0.6% Allyl alcohol , which was a selection agent. Mutants were
generated by exposing to UV radiations, with different exposure times and
stability is tested. The results were encouraging and the lactic acid produced
was about 23 g/l at temperature of 420C. The pH optimum was found to be about
5.2. and inoculum size was found to be 3 ml. The present studies were focused
on accumulation of lactic acid by using ground nut shells having ligno
cellulosic material after treatment with dilute acid. - See more at:
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