Glycoproteins are significantly contributing to
the surface properties of the cells. Glucosamine has membrane stabilizing
activity and liver protective effect. The aim of this study is to evaluate the
alteration of glycoprotein in hepatic fibrosis and the effects of glucosamine
administration in rats. The experiment was conducted into six groups of rats;
control, thioacetamide (TAA), glucosamine hydrochloride, TAA and glucosamine
hydrochloride, glucosamine sulphate and TAA and glucosamine sulphate groups.
Serum and liver carbohydrate moieties (total hexose, fucose, hexosamine, and
sialic acid) of the glycoproteins were evaluated in rats. Serum and liver
glycoproteins were isolated and analyzed by using sodium dodecyl sulphate
polyacrylamide gel electrophoresis. The results indicated that liver hexose and
sialic acid were lower in TAA rats administrated with glucosamine hydrochloride
or sulphate compared to TAA rats. Liver hexosamine of TAA rats administrated
with glucosamine sulphate was lower compared to TAA rats administrated with
glucosamine hydrochloride. Liver glycoprotein fucose showed a decrease in TAA,
TAA& glucosamine hydrochloride or sulphate groups compared to control
group. The isolated glycoproteins from serum showed high expression while
glycoproteins from liver showed low expression profile in TAA rats compared to
control and glucosamine administrated rats. In conclusion, the administration
of fibrotic rats with glucosamine hydrochloride or sulfate altered liver
glycoprotein especially rich in hexose and sialic acid.. - See more at:
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