The objective of this study was to evaluate the effects of two plants
rich in phenolics compounds on ruminal fermentation, methane production and
related microbial diversity usingin vitro gas production technique (IVGPT). 25
mg of the two plant extracts added to 200 mg of mixture of alfalfa hay,
ryegrass hay and corn (5, 3, 2) were incubated with 30 ml of buffered rumen
fluid (1: 2) in 120 ml serum bottles. After 24h, the incubation was stooped and
the inoculants were determined for pH, ammonia-N, VFA, truly organic matter
digestibility, methanogens and protozoa quantification using real time PCR
technique. In vitro gas production was recorded and methane concentration was
determined at 3, 6, 9, 12 and 24h of incubation. Results showed that methane
and ammonia nitrogen production was significantly reduced (p? 0.05), propionate
production was increased significantly (p? 0.05), while no significant effect
was registred for pH, tVFA and organic matter digestibility comparatively to
the control. Real time PCR indicated that the ciliate protozoa population in
the two added extracts was decreased (p? 0.05), while no effect was observed on
methanogens population. Among phenolics fractions, total flavonoids had the
closest relationship with CH4 production (r=-0.916, p ?0.01) followed by total
phenols (r=-0.861, p ?0.01) and condensed tanins (r=-0.538, p ?0.05). In
conclusion, the plants rich in flavonoids might be promising to be used as a
potent antimethanogenic additive for decreasing CH4while they obviously enhance
microbial biomass production and fermentation effeciency. - See more at:
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