. Background:- This research article reports that the production of
cellulases by Hypocrea nigricans; isolate of rotten wood barks. It was screened
for their efficiency in cellulose degrading enzymes particularly endo and exo-
?- 1, 4-D-glucanases on different lignocellulosic solid substrates. The aim of
the present study was to isolate a potent cellulolytic enzyme producing fungi
from natural habitat; optimisation of enzyme production by one factor at a time
(OFAT) strategy, then applied for purification and characterisation. Results:-
Among the screened lignocellulosic substrates, paddy husk has been identified
as the best source for maximum enzyme production and optimal cultural
conditions were recorded as pH 6.0, incubation temperature 30oC, moisture content
40%, spore density 2 X 106 spores/g of substrate. The production was increased
(3 folds approximately) from 3.9 U/g to 11.84 U/g under conventional
optimization studies. Each experiment was performed thrice, in each triplicate
mean value of experimental results were taken into consideration..The two
enzymes, endo and exo- ?- 1, 4-D-glucanases produced by Hypocrea nigricans were
purified and characterised by estimating their molecular weights as 54 and
48kDa respectively through SDS-PAGE. The purified cellulolytic enzymes was to
be accessible for biofuel, textile, paper pulp industrial exploitation
Conclusion:- The present study reveals the cellulolytic properties of Hypocrea
nigricans with optimised production. Maximising cellulolytic enzymes production
that will work under industrial conditions particularly with inexpensive, rapid
and sensitive testing methods. - See more at: .
No comments:
Post a Comment