Leaf curl disease in chilli is one of the major
constraints to the production of chilli (Capsicum annum L.) in the Indian
subcontinent which is caused by begomovirus and associated satellite. The
full-length genome of begomovirus and its cognate betasatellite DNA component
associated with chilli leaf curl disease (ChiLCD) originating from Bijnour,
Uttar Pradesh (U.P.), region of India were cloned and sequenced. The pairwise
nucleotide sequence identity shared <91% identity with DNA-A segment of Tomato
leaf curl New Delhi virus (ToLCNDV), while betasatellite DNA shared highest
nucleotide identity (>90%) with Tomato leaf curl Bangladesh betasatellite
(ToLCBDB). According to new species demarcation rules, we hereby, report
identification of a new begomovirus species infecting chilli plant in natural
conditions, and we propose to name as, Chilli leaf curl Bijnour virus. Head to
tail tandem repeat dimers of DNA-A and betasatellite DNA were prepared and
agroinoculated into chilli plant, which later showed slight curling and mosaic
development in leaves, thus fulfilling the Koch?s postulates. - See more at:
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