Objective:-The objective of present study was
to identify the unknown wild edible mushroom based on morphological
characteristics and DNA based sequencing by using universal primers ITS 1and
ITS 4, antibacterial activity, phytochemical screening and FTIR analysis of
mushroom extract spectra. Methods:- The wild edible mushroom specimen was
tentatively identified morphologically and was cultured on 2% MEA. The isolate
thus obtained was screened for its antimicrobial activity. Methanol extract was
prepared in order to carry out antibacterial activity. The extract was
subjected to qualitative phytochemicaland FTIR analysis. Results:-The isolates
on the basis of morphological and molecular characterization was identified as
P. ostreatus. Agar-well diffusion method was used to carry out antimicrobial
assay. The methanolextract ofP. ostreatus showed antimicrobial activity against
E.coli having zone inhibition diameter 24.3?0.5, against S. aureus having zone
inhibition diameter 26.6?0.5,againstP. aeruginosa having zone inhibition
diameter20.3?0.5 and against K.pneumoniae having zone inhibition diameter
28.6?0.5. Fourier transform infrared (FT-IR) analysis indicated that all
mushroom?s extract spectra showed typical carbohydrate pattern with strong and
broad absorption near 3300 cm-1which indicates the presence of OH group.
Conclusion:-The isolate analysis have been showed to be good source of
antimicrobial agent and phytoconstituents, therefore could be potential source
of bioactive compounds with beneficial biological activities. - See more at:
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