A TRITERPENOID SAPONIN FROM THE ROOT BARK EXTRACT OF Massularia acuminata spp.

Compound CFME2 was isolated as the second column fraction of chloroform soluble fraction of methanol extract of the root bark of Massularia acuminata and was identified to be a bioactive triterpenoid saponin. On the basis of chemical and spectroscopic analyses, the structure of the compound was established and the compound identified as 3-O – ?- D- glucopyranosyl – (1-4)- ?- D- glucopyranosyl – (1-4)- ?- D- glucopyranosyl – 28- O – ?- D- glucopyranosyl – (1-4)- ?- D- glucopyranosyl – (1-4)- ?- D- glucopyranosyl- oleanolic acid. 1D- and 2D- NMR experiments which include 1H, 13C, DEPT, 1H-1H COSY, HSQC and HMBC were used to achieve the structural elucidation. The compound showed good inhibitory ability against the microorganisms, Salmonella spp, Staphylococcus aureus, Escherichia coli, Aspergillus niger and Candida albicans. - See more at