BIOSYNTHESIS OF SILVER NANO PARTICLES FROM ENDOPHYTIC BACTERIA, ANTIBACTERIAL ACTIVITY AND MOLECULAR CHARACTERIZATION OF Bacillus subtilis.

This study highlights the ability of nitrate reducing Bacillus subtilis STBBP1 cell free extract used for the preparation of silver nanoparticles . The SEM graph revealed spherical shaped Nanoparticle in the range of 40-60mm. The antibacterial activity of silver Nanoparticles by Bacillus was investigated against various pathogenic organisms. Highest antibacterial activity was found in Escherichia coli (15mm) followed by Streptococcus pyogenes (11mm). The formation of silver Nanoparticles was monitored by UV-Vis spectra showed surface Plasmon resonance peak at 430 nanometer, SEM-EDX spectra showed the presence of element silver in pure form. FTIR proved the the presence of biomolecule responsible for the reduction of silver ions and X-ray diffraction analysis confirmed that the obtained silver Nanoparticles were in crystalline form. It showed an array of absorbance bands in 600cm -1. The molecular characteristic of Bacillus subtilis STBBP1 was evaluated by PCR amplification of 16srRNA and sequences were deposited in gene bank . This opens a new avenue of result were the Endophytic bacteria can also be used in the synthesis of Nanoparticles. -See more