ISOLATION, IDENTIFICATION AND CONTROL OF BACTERIA AND FUNGAL MICROORGANISMS FROM CONTAMINATED CURRENCY NOTES. - See more at: http://www.journalijar.com/article/8353/isolation,-identification-and-control-of-bacteria-and-fungal-microorganisms-from-contaminated-currency-notes./#sthash.YwtwTzeS.dpuf

Money is very important to human life as it facilities the needs and currency notes are vital for goods and services worldwide. Paper currency is used repeatedly in exchange for goods and service and this are way the circulation of paper currency from one individual to another potentially spreads microorganisms. Contaminated different paper currency note samples were collected from hospital in Tiruchirappalli,Tamilnadu. The samples were analysed in microbiologically. Both gram positive and gram negative bacteria were found on currency notes. Predominant bacteria found in 25 currency notes were Streptococcus pneumonia present (36%), Bacillus subtilis (24%), Pseudomonas aeruginosa (18%), Escherichia coli (12%) and Klebsiella pneumonia (10%). fungi were Aspergillus flavus (4%), Aspergillus fumigatus (8%), Aspergillus niger (4%) and Candida albicans (8%). DNA was separated by Agarose Gel Electrophoresis. The size of the DNA measured using molecular marker. The bands found at 9500 bp and 8000 bp respectively. The sensitivity tests were performed to detect the sensitivity of organisms against Standard disc placed. The maximum zone was observed in Escherichia coli and Streptococcus pneumonia against commercial antibiotics such as Chloramphenicol, Erythromycin .In fungi, the maximum and minimum zone of inhibition was observed in antibiotic clotrimazole and Amphotericin B respectively. The maximum level of inhibition was present in 20 minutes UV treatment. Paper currency is commonly contaminated with microbes and this may play a role in the transmission of potentially harmful organisms. So cash should not be handled by children and should be kept away from food and cosmetics. - See more at: