SYNTHESIS AND STUDY OF ANTIMICROBIAL & ANTI-OXIDANT PROPERTIES OF SUBSTITUTED DERIVATIVES OF COUMARIN.

Coumarins and their derivatives are very important organic compounds; they are biologically active and widely occur in nature1,2Recent studies have been revealed that coumarin and the derivatives exhibit several other medicinal applications such as anti-coagulants, antifungal, insecticidal, hypnoticsphytoalexins, HIV protease & inhibitors3. Thus the synthesis of coumarins is of continuing interest. Potassiumdihydrogen phosphate a commercially available environmentally benign catalyst non-toxic widely used for the synthesis of the substituted coumarin4 .The scope of this catalyst has not been fully explored, but can be used as buffer, neutralizing agent. Owing to the numerous advantages associated with cheap and non-hazardous catalyst, and also realizing g the importance of coumarin herein we would like to focus the eco ?friendly method for his synthesis of derivatives of coumarin using cheaper and commercially available acid catalysts Potassium dihydrogen phosphate and also by the Knoevenagel condensation under microwave irradiation. The synthesized coumarin derivative was screened in Vitro anti-microbialefficacy testing and anti oxidant properties. In vitro anti-microbial efficacy testing was carried out by broth dilution method by broth dilution method as mentioned in ?Pharmaceutical Microbiology?. For anti-bacterial activity, MullerHinton medium was used as the nutrient media. Test bacterial species used are Escherichia coli ,(ATCC 10148), Staphylococcus aureus(NCTC 3750), Pseudomonas aeruginosa (Fisher?Immunotype IV), test fungi species used are Aspergilliusniger(ATCC 16404) and Candida albicans (ATCC 10231) in different concentrations starting from 25ppm .All the coumarin derivatives are active against the test bacteria and fungai in different concentrations. Anti-oxidant studies of all these derivatives have been carried out . These compounds were characterized by IR, NMR spectra?s. This paper focuses is to develop environmentally reactions, simple, highly efficient and high yielding protocol for the synthesis of coumarin derivatives using Potassiumdihydrogen phosphate as a catalyst. Therefore owing the importance of Potassium dihydrogen phosphate a facile catalyst used for the green synthesis of new derivatives of coumarin. - See more at: