Synthetically chemical fungicides have long been used to reduce the
incidence of plant diseases. However they are costly, can have negative effects
on the environment and may induce pathogens resistance. The application of
chemical compounds is consider as the most in expensive and common method in
plant disease control. Therefore efficacy of leaves extracts of Annona squamosa
Linn, Brassica campestris Linn. (Sarson) and Ocimum sanctum Linn against
Sclerotium rolfsii was studied. The in vitro antifungal activity of these
Annona squamosa Linn, Brassica campestris Linn. (Sarson) and Ocimum sanctum
Linn leaves extract was investigated. The medicinal plant leaves extract of
10%, 25%, 50%, 75% and 100% (without treatment serve as control) in aqueous
medium were select for made. Inhibited values recorded. In the case of Brassica
campestris Linn (Sarson) 8.534, 9.302, 11.24 and 10.86 percentage of inhibition
recorded at 10, 25, 50 and 75 percent concentration after 72 hours incubation
periods. The Annona squamosa Linn leaves gave 21.33mm mycelial growth at 25
percent concentration of with 3.045 percent of inhibition 77.33mm mycelial
growth at 50 percent aqueous concentration gave 3.337 least percentage of
inhibition. The Ocimum spp. revealed 32.00 and 30.68 percentage of inhibition
with 17mm and 17.33mm mycelial growth at 50 & 75 percentage concentration
of Ocimum spp. aqueous leaves extract after 24 hours. The maximum inhibition
was 74.33mm and 73.33mm at 25 percent and 50 percent of Ocimum spp. aqueous
leaves extract after 72 hours of incubation period. All used concentration of
three medicinal plant leaves extracts effectively suppress the radial mycelial
growth of Sclerotium rolfsii ranging from 2.197% of inhibition in Ocimum spp.
at 25% of concentration after 72 hours, to 32.00% of inhibition in same Ocimum
spp. leaves extract at 50% concentration leaves aqueous leaves extract after 24
hours incubation periods. However 0% gave by Brassica compestris leaves extract
of 10 & 25 concentration after 24 hours of incubation period. The present
study was conducted with an aim of determining antifungal activity of medicinal
plant leaves extract of Annona squamosa, Brassica campestris Linn. (Sarson) and
Ocimum sanctum Linn. - See more at: .
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