GENETIC VARIATION OF TRICHOMONAS VAGINALIS ISOLATES FROM IRAQI WOMEN: A NEW SEQUENCE TYPE - See more at: http://www.journalijar.com/article/8293/genetic-variation-of-trichomonas-vaginalis-isolates-from-iraqi-women:-a-new-sequence-type/#sthash.bqnxjnnd.dpuf

The Trichomonas vaginalis genome was found to be approximately 160 megabases in size and the developed genotyping tools allow better understanding of its population genetics. This study aimed to determine the genetic variation among T.vaginalis isolates by Multilocus sequence typing (MLST) method for six housekeeping genes. A total of 154 Iraqi women (age range 15-54 years) who were attending the Gynecology out -patient department in Baghdad province during the period from February 2013 to April 2014, were recruited for this study. High vaginal swabs were collected then diagnosed the infection by culturing method. DNA of T.vaginalis was extracted from the cultured swabs. MLST method for six housekeeping genes, were used for studying the genetic variations among T.vaginalis isolates. Fifty three (34.41%) women were positive for T.vaginalis. MLST method for housekeeping genes resulted in different alleles and there was a new allele in four loci based on the database of GenBank. Furthermore, a new sequence type of T.vaginalis was found based on the Trichomonas MLST website. It can be concluded that there are a wide genetic diversity among local T.vaginalis isolates which influence the clinical outcome, and their local strains have a characteristic allele compared with a universal - See more at: