Crude alpha-hemolysin was produced by Staphylococcus aureus and it was
extracted from the same bacteria which was isolated from clinical samples
collected from skin tissue infection and sepsis.the hemolytic activity of crude
alpha-hemolysin against rabbit erythrocytes was determined. The culture
supernatant was treated with ammonium sulfate (75% saturation)and also
hemolytic activity after addition of ammonium sulfate was measured. Skin test
was determined against rabbit to detect its ability to induce delayed type
hypersensitivity sensitivity and it gave positive results in compare with
control and lethal toxin activity was tested in mice to reveal its ability to
induce infection symptoms and mortility after 48 hour and 18 hour. so this
study proved that crude alpha-hemolysin possess properties enable it to
behavior as antigen and to be effective virulence factor in S. aureus. - Seemore at:
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